ASSESSMENT OF ANTIMICROBIALACTIVITIES OF IWGELLA SATINA SEED EXTRACTS AGAINST CARBAPENEM-RESISTANT CLINICAL BACTERIAL ISOLATES

Abstract

areresistancetothe antibioticsthatarecommonly‘usedfortheirtreatment.Ther"efore,thereis

theneedto developasafeandeffectivealternativeto combattheinfections causedbythese multipledrugresistantbacteria.Thisstudyaimedatassessingtheantimicrobialactivitiesof. tbreedifferentextractsof\'pe/lasofiv‹iseedagainstcarbapenem-resistantclinicalbacieria'l"' isolates.Thirty-three(33)bacterialisolatesfromdifferentclinicalsamples(blood,sputum,’ wound scab. earscab, urine,stool, centrallineswab) used in thisst (id\’’iveteobtainedtroiii threedifferentsolventswhichweien-hexanc,iiiethanolandsteriledistilledPatelusing Jacceleratedsolvent extractionmethod. "I'lie c-ritde extracts ofthe .X\.sariveeds were subjecte‹i. tc›qualitativephytochemicalscreeningusingstandardmethods.Gas-ChromatographyPass-Spectrophotometry(GC-MS)analysiswasalsOcaiTiedouttoidentimajorphytochemicals andfattyacidsintheextracts.Theantibioticsusceptibilitytestsandminimum*’inhibitory’ concentration(UC) etcdeterminedusingagarwelldiffusionandserialdilution"m‹ithods: “ respectively.Datawereanalyzedusinyone-ayanalysisofvariancewith P<0.05considered statisticallysignificant.Thepotencyofthe.â*..safiv‹iextracts»asassessedusingmolecular docking.Fromthethirty-three(33)testedisolatesobtained.54.5%(n=l8)wereresistanttoone or worecarbapeneliiantibiotics; 39.4% (n=13), 36.4% (n—12) and 45.5% (n=l5) were resistant toiirtipencm,meropcficiTiandertapencm,rcspcctitelf’.F'rcmthePCkresult.5.1.s%ofthe isolates carried one or two of the carbapenemasegenes. T“he aqueous extract Sf.â. .sativa seed showednotoneofinhibitionagainstthetestedisolates.Then-hexaneextracthadzoneof

MicrobiologIdiaSnosticlaboratoriofBabcockUnivers-ityTeachingho.s.pital*lli:san-remo* inhibitionthatrangedfrom26+3mmto6+4mmwhilethemethanolicextracthadzoneof inhibitionfrom124mmto6-£3mm.TheMICrangedfrom40mg/mLto120mg/mL.The qualitativepliytochemicalscreeningindicatedthepresenceofcardiacglycosides,steroids, phenols in all three extracts. Other phytocheinicalspresent included alkaloids, saponins, terpenoidsandflavonoids(methanolextract).tannins,alkaloidsandterpernoids(n-hexane extract) and flavonoids (aqueous extract). The GC-MS analysis revealed the presence of differentfattyacidswhichinclude9,12-Octadecadienoicacid.Octadecanoicacid.i-lexadccaooic °.cid. Cyclopropane,l,l-dichloro-2,2.3.3-tctramethyl, Methyl s:cara:e. PropyleneglycolMonoleateand11,13-Eicosadienoicacid.TheGC-MSresultswhendocked withthepenicillinbindingproteinofthebacteriarevealedPropyleneglycolSlonoleaietoliaxe thehighest bindingnegativeenergy.Thisstudy suggeststhat1S’igellasativaseedextracts (methanoland n-hexane) possess antimicrobialactivities against carbapenemresistantbacteria.