College of Biosciences
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Item INFLUENCE OF RHIZOSPHERIC BACTERIA 0N GROWTH AND YIELD OF TOMATO (Solanum lycopersicum L.) UNDER WATER AND DISEASE STRESS CONDITIONS(2023-01-20) TAIWO, MICHAEL OLUWAMBEItem PRODUCTION AND CHARACTERIZATION OF CELLULASES FROM BACILLUS SPECIES AND ITS APPLICATION ON BIOPULPING AND EXTRACTION OF ORANGE PEEL ESSENTIAL OIL(2025-10-15) OLAITAN, CATHERINE OHUNENEItem THE USE OF SELECTED BOTANICALS AND NANOPARTICLES TO CONTROL TOXIGENIC MOULDS ASSOCIATED WITH STORED COCOA BEANS(2023-03-20) OGUNDEJI, BABATUNDE AYODEJIItem IMMOBILISATION OF CELLULASE SYNTHESIZED BY Aspergillus species WITH Persea americana MATRIX AND ITS APPLICATION IN PALM OIL EXTRACTION(2025-10-20) JEJELOLA, TOLUWALOPE JAMESItem SILYMARIN AS A POTENT INHIBITOR OF AKT PATHWAY IN N-' NITROSODIETHYLAMINE- INDUCED HEPATOCELLULAR CARCINOMA IN MALE WISTAR RATS(2025-07-20) KAMARUDEEN ADEWUMI AREMUItem BIOSYNTHESIS OF SECONDARY METABOLITES BY Streptomyces spp. AND THEIR ANTI-ULCER ACTIVITY AGAINST ULCER-ASSOCIATED ORGANISMS(2025-09-20) ZAKARI, ADEIZA DAVIDItem STARTER CULTURE DEVELOPMENT AND FERMENTATION OF OREOCHROMIS NILOTICUS (NILE TILAPIA ) PREPARED WITH DIFFERENT MARINATING AGENTS(2025-07-22) ABIODUN SUNDAY OLUSEGUNItem ASSESSMENT OF ANTIMICROBIAL ACTIVITES OF NIGELLA SATIVA SEED EXTRACT AGAINST CARGAPENEM-RESISTANT CLINICAL BACTERIAL ISOLATES(2025-09-18) ADIO, IYABODE TOYINItem IMMOBILISATION OF CELLULASE SYNTHESIZED BY Aspergillus species WITH Persea americana MATRIX AND ITS APPLICATION IN PALM OIL EXTRACTION(2025-10-29) JEJELOLA, TOLUWALOPE JAMESItem OPROSPLECTION OFANTIBACTER1AL ACTIVITIES OF CINNAMON AND CLOVE CRUDE EXTRACTS AND ESSENTIAL OILS AGAINST BACTERIA ISOLATED FROM INFECTED SURGICAL SITES(2025-09-23) ALAKE, AANUOLUWAPO OLAMIDEItem ANTIBACTERIAL POTENTIALS OF ENDOPHYTIC FUNGAL METABOLITES FROM MEDICINAL HERBS; Caries papaya, Mangifera indica And Gossypium hirsutum ON PERIODONTAL ISOLATES.(2025-08-25) AKINKUNM1, OPEYEMI BAMIDELEItem STARTERCULTURE DEVELOPMENT AND FERMENTATION OF OROCHROMISNILOTICIS (NILETILAPIA) PREPARED WITH DIFFERENT MARINATING AGENTS(2025-07-16) ABIODUN,SUNDAY OLUSEGUNABSTRACT Fermentation of fish is meant to extend the shelf life of fish, improve sensory characteristics and enable consumers to access benefiting microorganisms. Dried fermented fishier stapling many cuisines, particular in coastal areas. However, discrepancies in fermentation methods and sensory quality have as substantial impaction consumer's acceptability and food safety. This research aimed to screen for possible isolates that could be used as starter cultures during spontaneous fermentation of Oreochromis niloticus identify the best isolates using morphological, biochemicalandmoleculartechniques,fermentwiththeselectedisolatesandtodetermine sensory acceptability of dried fermented fish. Marinated fish were spontaneously fermented at 10‘Cfor12daystoisolatestarterculturesanddeterminebestfermentationperiod.Coloniesof bacteria isolated with MRS (de Man Rogosa and Sharpe agar) plates during spontaneous fermentation of Oreochromisniloticus were characterized, identified and used as starter cultures. Fermentedfishwereovendriedat60’Cfor 12hours. Proximate analysis of dried fermented fish was done using standard methods. The appearance, aroma, taste, tactile texture, overall acceptabilityoffermentedfishwereevaluated.Datawereanalyzedusingone-wayanalysisof variance, followed by Duncan's multiple range test, Pñ0.05 was considered significant. Results of fermentationperiodonphysicochemicalparametersrevealed10daysfermentationperiodat10 ‘*was the best as protein content increased from initial 23.05 to 29.03 in com base fermented fish and23.86to30.83incassavabasefermentedfish.Suspectedcontaminatingorganismswere completely inhibited bytheactivitiesofthestartercultures;thesystembecameslightlyacidic with Total bacteria count (TBC)7.8^109/cfu and Lactic acid bacteria (LAB) 3.1•10'/cfu for com base while for cassava base, TBC-1.2•109/cfu and LAB-2.2•105/cfu for cassava base. Comparativeproximateanalysisoffreshfish,fermentedfishanddriedfermentedfish showed protein content of(24.12, 31.92, 30.04 %), Fat content of(2.69, 2.16, 1.88 %), and Ash content of (5.82,7.23,9.12%), respectively. The main and interactive effect so base and isolate Sensory acceptability showed that carbohydrate bases had no significant (P>0.05)effect on fermented fish acceptability. Starter cultures had significant effect on taste (P= 0.004) and aroma (P=0.009).Combinedeffectofcarbohydratebaseandstartercultureshadsignificanteffecton appearance(P=0.002)and aroma(P= 0.004). Sensory attributes revealed that the effects of the three starter cultures on the fermented fish were not significantly different (P>0.05). The selected bacteriaisolateswereidentifiedasLactobacilluspentosusstrain,Streptococcusthermophilusstrain1,and Streptococcus thermophilus strain2,respectively. Dried fermented fish had improved values compared with fresh fish in Na (62.80+3.4vs.52.50z2.3),and Fe (41.90+6.7) mg/100g. This study demonstrates the potential of these developed stains as starter cultures to improve the quality and nutritional value of fermented Oreochromis niloticus.Item EFFECT OF CHITOSAN SUPPLEMENTATION ON1,2- DIM ETHYLHYDRAZINE- INDUCED INTESTINAL DYSBIOSIS AND HEPATOXICITY IN MALE WISTARN RATS(2025-10-18) ADEBIYI, VICTORIAAYOMIDEItem BIOPROSPECTIONOFANTIBACTERIALACTIVITIESOFCINNAMONANDCLOVE CRUDEEXTRACTSANDESSENTIALOILSAGAINSTBACTERIAISOLATED FROMINFECTEDSURGICALSITES(2025-09-27) ALAKE,AANUOLUWAPOOLAMIDEABSTRACT Antibioticresistanceespeciallyinsurgicaltreatmentsisamajorglobalhealththreat.Thiscallsfor urgency in investigating sustainable antibacterial agents from alternative sources. This study investigatedtheantibacterialactivityofCinnamomumcassia(cinnamon)andSyzygiumaromaticum(clove)crudeextractsandessentialoilsagainstbacteriaisolatedfrominfectedsurgicalsites.Eight woundswabsampleswereobtained frompatientsclinicallydiagnosedwithsurgicalsiteinfection and cultured on nutrient agar plates using standard isolation procedures. Bacterial isolates were identifiedviamorphological,biochemical andmolecularmethods.Essentialoils(EOs)andextracts ofcinnamonandclovewereobtainedviahydrodistillationandmaceration,respectively.Antibiotic sensitivity testoftheisolatesagainstsixclassesofantibiotics wascarried outusingKirbyBauer's discdiffusion method.Sensitivity oftheisolatestotheEOs,extractsaswellastheircombinations weredeterminedusingagarwelldiffusionmethod.MinimumInhibitoryConcentration (MIC)and Minimum Bactericidal Concentration (MBC)oftheessentialoils,extractsandtheircombinations againsttheisolatesweredeterminedusingdouble-folddilutionmethod.RateofkilloftheEOsand extractsagainstmostsusceptibleisolateswasevaluated.Phytochemical propertiesoftheessential oilsandextractswereevaluated usingGasChromatographyandMassSpectrometry.Insilicostudy wascarriedoutonthebioactive compoundsoftheEOsand extractsusingiGEMDOCKandSWISS-ADME.BacteriaisolatedincludeEscherichiacoli(20%),Proteusvulgaris(10%),Staphylococcus epidermidis(20%),Moraxellacatarrhalis10%),ProteusmirabilisI0%),Vibrioparahemolyticus(10%),Klebsiella aerogenes (10%),Klebsiella quasipneumoniaesubsp.similipneumoniae(10%) and Staphylococcus saprophyticus (10%). Antibiotic sensitivity test revealed all isolates to be multidrug-resistant.Allisolatesdemonstrated susceptibilitytotheEOsandextracts,withzonesof inhibitionranging from17.0z0.0mmto50.0+2.3mmforcinnamonEOs andextracts, 10.0z0.0mmItem ENTOMOTHERAPEUTICPOTENTIAL OFVOSR!••ulgaris MUD NEST AND VENOM AGAINST MULTIDRUG-RESISTANT BACTERIA IMPLICATED IN UPPER RESPIRATORY TRACT INFECTIONS(2025-09-17) AKINSOWON,TEMILADEKINGSLEYABSTRACT Theincreaseinmultidrug-resistant(MDR)bacteriaposesasignificantchallengeintreatingupper respiratorytractinfections(URTIs).ThisstudyinvestigatedthepotentialofVespulavulgarismud nest extract and venom as alternative treatment against multidrug-resistant bacterial strains associatedwithupperrespiratorytractinfections.Onehundredsputumsampleswerecollectedfrom patientsattheFederalMedicalCenter,Idi-Aba,Abeokuta,OgunState.Isolationofbacteriawas performed using nutrient agar, blood agar, and MacConkey agar. Bacteria were isolated using standardmicrobiologicalmethodsandwereidentifiedusingphysiological,morphological andIn-silico molecular docking. Kirby Bauer's disc diffusion testwas used todetermine the antibiotic sensitivityprofileswhileagarwelldiffusionwasusedtodeterminethesusceptibilityoftheisolates to the mud nest extract and venom. Minimum Inhibitory Concentration (MIC) and Minimum BactericidalConcentration (MBC) weredetermined.Theeffectofthecombinationofthemudnest extractandvenomwithcommercialantibioticswasevaluatedusingoverlayinoculumsusceptibility technique.Physicochemicalanalysisofthemudnestextract gottenthroughethanolic extraction whilethevenomwasgottenfromP'espulavulgarisabdomenwiththeuseofneedleandsyringeand was aseptically dispensed inanEppendorf tube. The chemical compounds in the mud nest and venomweredeterminedusingGasChromatographyandMassSpectrometry(GC-MS)andwere evaluated for their pharmacokinetic properties using in silico methods. One-way Analysis of Variance (ANOVA) was used in analyzing the data. Klebsiella pneumoniae, Escherichia coli,Pseudomonasaeruginosa,Staphylococcusaureus,Bacillussubtilis,StreptocoCcttspneumoniae, Klebsiellaoxytoca,andProteusmirabiliswereidentifiedthroughtheBergy'sManualofSystematic Bacteriology.Antibioticsusceptibilitytestingresultsrevealedmultidrugresistancewhileagarwell diffusionshowedazoneofinhibitionrangeof18.2z0.00mm—25.5z0.00mm.MICof3.125mg/ml — 25mg/mland6.25mg/ml—50mg/mlwereobserved formudnestandvenom,respectively. MBC ofmudnest was between12.5mg/ml —50mg/ml while venom showedMBC of12.5mg/ml—100 mg/ml. Gas chromatography and mass spectrometryanalysisindicatedthe presenceofDecane,2,6,7 — trimethyl, undecane, decane among others for Vespula vulgaris mud nest and 3,6-bis(N-dimethylamino)-9-ethylcarbazole,Arsenous(trimethylsilyl)ester, Trifluorpaceticacidandcarbonic acidforthevenom. In-silicomolecular docking showed dibutyl phthalate andhexadecenoicacidas potentialleadmoleculeinthedevelopmentofnewdrugtocombatbacteriaimplicatedinURTIs. Thisstudycontributestothegrowing bodyofknowledgeonalternative therapiesandhighlights the needforongoingresearchtoaddressthechallengesposedbyantibioticresistanceinclinicalsettings. The investigation from this study showed Vespula vulgaris mud nest and venom possess significant antibacterialpotencyagainstmultidrug-resistantbacteriacommonlyfoundinupperrespiratorytract infections. The results in this study support the potential of these natural substances as a viable alternative therapyfortreatinginfections caused bythesechallenging pathogens.
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