College of Biosciences

Permanent URI for this collectionhttps://ir.funaab.edu.ng/handle/123456789/251

Browse

Browsing College of Biosciences by Author "ALAKE,AANUOLUWAPOOLAMIDE"

Filter results by typing the first few letters
Now showing 1 - 1 of 1
  • No Thumbnail Available
    Item
    BIOPROSPECTIONOFANTIBACTERIALACTIVITIESOFCINNAMONANDCLOVE CRUDEEXTRACTSANDESSENTIALOILSAGAINSTBACTERIAISOLATED FROMINFECTEDSURGICALSITES
    (2025-09-27) ALAKE,AANUOLUWAPOOLAMIDE
    ABSTRACT Antibioticresistanceespeciallyinsurgicaltreatmentsisamajorglobalhealththreat.Thiscallsfor urgency in investigating sustainable antibacterial agents from alternative sources. This study investigatedtheantibacterialactivityofCinnamomumcassia(cinnamon)andSyzygiumaromaticum(clove)crudeextractsandessentialoilsagainstbacteriaisolatedfrominfectedsurgicalsites.Eight woundswabsampleswereobtained frompatientsclinicallydiagnosedwithsurgicalsiteinfection and cultured on nutrient agar plates using standard isolation procedures. Bacterial isolates were identifiedviamorphological,biochemical andmolecularmethods.Essentialoils(EOs)andextracts ofcinnamonandclovewereobtainedviahydrodistillationandmaceration,respectively.Antibiotic sensitivity testoftheisolatesagainstsixclassesofantibiotics wascarried outusingKirbyBauer's discdiffusion method.Sensitivity oftheisolatestotheEOs,extractsaswellastheircombinations weredeterminedusingagarwelldiffusionmethod.MinimumInhibitoryConcentration (MIC)and Minimum Bactericidal Concentration (MBC)oftheessentialoils,extractsandtheircombinations againsttheisolatesweredeterminedusingdouble-folddilutionmethod.RateofkilloftheEOsand extractsagainstmostsusceptibleisolateswasevaluated.Phytochemical propertiesoftheessential oilsandextractswereevaluated usingGasChromatographyandMassSpectrometry.Insilicostudy wascarriedoutonthebioactive compoundsoftheEOsand extractsusingiGEMDOCKandSWISS-ADME.BacteriaisolatedincludeEscherichiacoli(20%),Proteusvulgaris(10%),Staphylococcus epidermidis(20%),Moraxellacatarrhalis10%),ProteusmirabilisI0%),Vibrioparahemolyticus(10%),Klebsiella aerogenes (10%),Klebsiella quasipneumoniaesubsp.similipneumoniae(10%) and Staphylococcus saprophyticus (10%). Antibiotic sensitivity test revealed all isolates to be multidrug-resistant.Allisolatesdemonstrated susceptibilitytotheEOsandextracts,withzonesof inhibitionranging from17.0z0.0mmto50.0+2.3mmforcinnamonEOs andextracts, 10.0z0.0mm